Cartalax (20mg) Research Peptide: An AED Tripeptide for Cartilage, Joint & Musculoskeletal Studies

If your research investigates cartilage biology, joint health, or the molecular mechanisms of connective tissue repair and cellular aging, Cartalax (20mg) research peptide — also known as the AED tripeptide (Ala‑Glu‑Asp) or T‑31 — offers a precisely defined synthetic tool with a growing body of scientific literature.

Cartalax is a synthetic tripeptide bioregulator (Ala‑Glu‑Asp; AED sequence) developed through research on cartilage tissue extracts. It is a short peptide bioregulator designed to target cartilage tissue and is part of the Khavinson peptide family developed in Russia for longevity research. Its structural alignment with sequences found in the alpha‑1 chain of type XI collagen suggests potential interactions with extracellular matrix components. Studies demonstrate Cartalax modulates cellular aging pathways by reducing senescence markers p16, p21, and p53. Research also shows that Cartalax increases cell proliferation rates and beneficially alters the levels of various cytokines.

This makes Cartalax (20mg) research peptide a valuable tool for laboratories investigating cartilage repair, osteoarthritis, connective tissue biology, extracellular matrix (ECM) dynamics, and the fundamental processes of cellular aging.

At Chinese Grey Peptides, we supply high‑purity Cartalax (20mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to >98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into cartilage repair, joint health, and connective tissue biology begin with uncompromising quality.

What Is Cartalax and How Does It Work?

To fully appreciate the research potential of Cartalax (20mg) research peptide, it is essential to understand its biochemical identity and the mechanisms by which it exerts its effects.

The AED Tripeptide

Cartalax is a synthetic tripeptide consisting of three amino acids: alanine, glutamic acid, and aspartic acid — the sequence Ala‑Glu‑Asp (AED). Its molecular formula is C₁₂H₁₉N₃O₈, and its molecular weight is approximately 333.29 g/mol.

The peptide’s sequence is derived from research on cartilage tissue extracts and is structurally aligned with sequences found in the alpha‑1 chain of type XI collagen. Cartalax is one of the Khavinson family of short peptide bioregulators — molecules designed to mimic naturally occurring regulatory peptides involved in tissue homeostasis. It is specifically designed as a cartilage and musculoskeletal system bioregulator.

Mechanism of Action: Multi‑Target Bioregulation

The biological activity of Cartalax (20mg) research peptide is mediated through several intersecting mechanisms at the cellular and molecular level:

Chondrocyte Gene Regulation and Matrix Synthesis

Cartalax is proposed to bind DNA regulatory sequences in chondrocytes, upregulating collagen type II (COL2A1) and aggrecan (ACAN) gene expression. This increases production of cartilage extracellular matrix components — collagen II and aggrecan — while simultaneously exerting anti‑catabolic effects. Cartalax regulates the synthesis of proapoptotic factors and modulates matrix metalloproteinases (MMPs) to promote type I and type II collagen synthesis, helping maintain extracellular matrix integrity.

Cellular Proliferation and Anti‑Apoptotic Effects

Cartalax increases Ki‑67 and CD98hc expression, both of which decline during cellular aging. It decreases p53 signalling and suppresses caspase‑mediated apoptosis, reducing cell death. Research shows Cartalax increases cell proliferation rates and beneficially alters the levels of various cytokines. It has been shown to reduce senescence markers p16, p21, and p53.

Anti‑Inflammatory and Immunomodulatory Effects

Cartalax has demonstrated efficacy in animal models of osteoarthritis and in older patients with OA, helping regulate inflammatory pathways. It reduces joint pain and inflammation associated with conditions such as osteoarthritis and rheumatoid arthritis. The peptide also modulates cytokine levels and reduces inflammation.

Fibroblast and Connective Tissue Support

Cartalax has a widespread systemic impact due to its effects on fibroblasts. It has been shown to benefit skin and other connective tissues as well as the kidneys, and helps regulate the function of cartilage and skin fibroblasts.

Key Research Applications for Cartalax (20mg)

Cartalax (20mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Cartilage & Osteoarthritis Research

As a peptide that upregulates genes involved in cartilage matrix synthesis and downregulates genes associated with cartilage degradation, Cartalax is a valuable tool for studying osteoarthritis and cartilage biology. Research applications include:

• Chondrocyte Gene Regulation: Investigating how Cartalax modulates COL2A1 and ACAN gene expression.

• Collagen and Proteoglycan Production: Studying the peptide’s ability to support the synthesis of type II collagen and aggrecan.

• Cartilage Degradation: Exploring how Cartalax modulates MMPs to preserve cartilage integrity.

• Osteoarthritis Models: Investigating the peptide’s effects in animal models of OA and in older patient populations.

2. Fibroblast & Connective Tissue Research

Cartalax’s effects on fibroblast proliferation and apoptosis make it a valuable tool for studying connective tissue biology. Research applications include:

• Fibroblast Proliferation: Investigating how Cartalax enhances Ki‑67 and CD98hc expression and promotes fibroblast growth.

• Apoptosis Studies: Exploring how Cartalax suppresses caspase‑mediated apoptosis and reduces p53 signalling.

• ECM Remodelling: Studying how Cartalax modulates MMP balance to preserve ECM integrity.

• Cellular Senescence: Investigating the peptide’s ability to reduce p16, p21, and p53 expression.

3. Cellular Aging & Senescence Research

Cartalax has demonstrated effects on markers associated with cellular senescence, making it a valuable tool for aging research:

• Senescence Markers: Studying how Cartalax reduces the expression of p16, p21, and p53.

• Cellular Proliferation: Investigating how Cartalax increases Ki‑67 and CD98hc expression.

• Anti‑Apoptotic Effects: Exploring how Cartalax suppresses caspase‑mediated apoptosis.

• Cytokine Modulation: Studying how Cartalax alters levels of various cytokines.

4. Inflammation & Immunomodulation Research

Cartalax’s anti‑inflammatory properties make it a valuable tool for studying inflammatory signalling:

• Inflammatory Signalling: Investigating how Cartalax reduces joint pain and inflammation in OA models.

• Cytokine Modulation: Studying the downregulation of pro‑inflammatory cytokines.

• Immunomodulation: Exploring the peptide’s broader immunomodulatory effects in cartilage and connective tissue.

5. Tissue Regeneration & Matrix‑Peptide Interaction Research

Cartalax is used in tissue‑regeneration modelling and peptide‑therapy mechanistic work. Research applications include:

• Extracellular Matrix Homeostasis: Investigating how Cartalax maintains ECM integrity through collagen synthesis and MMP modulation.

• Cartilage and Connective Tissue Repair: Studying Cartalax’s potential to promote regeneration.

• Matrix‑Peptide Interaction Analysis: Exploring the peptide’s interactions with extracellular matrix components.

Chemical & Physical Specifications for Cartalax (20mg)

Accurate characterisation of Cartalax (20mg) research peptide is essential for reproducible experimental design.

Reconstitution & Handling Protocol for Cartalax (20mg)

Proper reconstitution of Cartalax (20mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Cartalax vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Cartalax vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 20 mg per 2 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Cartalax into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for Cartalax (20mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for Cartalax (20mg)

Why Choose Chinese Grey Peptides for Cartalax (20mg)?

As an authentic licensed peptide supplier based in the USA, we supply Cartalax (20mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed >98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Cartalax (20mg) Research Peptide

What is Cartalax (20mg) research peptide?
Cartalax (AED, T‑31) is a synthetic tripeptide (Ala‑Glu‑Asp) belonging to the Khavinson family of peptide bioregulators. It is derived from cartilage tissue research and is used in studies of cartilage biology, connective tissue repair, inflammation, and cellular aging.

 What is the sequence and molecular weight of Cartalax?
The sequence is Ala‑Glu‑Asp (AED). Its molecular formula is C₁₂H₁₉N₃O₈, and its molecular weight is approximately 333.29 g/mol.

What are the primary research applications for Cartalax?
Cartalax is used in cartilage and osteoarthritis research (chondrocyte gene regulation, collagen and aggrecan production), fibroblast and connective tissue research (proliferation, apoptosis, ECM remodelling), cellular aging and senescence studies, inflammation and immunomodulation research, and tissue regeneration and matrix‑peptide interaction studies.

What is the mechanism of action of Cartalax?
Cartalax is proposed to bind DNA regulatory sequences in chondrocytes, upregulating collagen type II (COL2A1) and aggrecan (ACAN) gene expression. It increases Ki‑67 and CD98hc expression, decreases p53 signalling, suppresses caspase‑mediated apoptosis, and modulates MMP balance to promote collagen synthesis and maintain ECM integrity.

 What purity does your Cartalax (20mg) meet?
Our Cartalax is tested to >98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

 How should I reconstitute Cartalax (20mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

How should I store reconstituted Cartalax?
Reconstituted Cartalax should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Cartalax research peptide legal to purchase in the USA?
Yes. Cartalax is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: Cartalax has been developed in Russia and has been studied in preclinical settings; it is not registered as a medicinal product with regulatory agencies such as the FDA, MHRA or EMA.

 Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

1. Shop Page
2. Bulk Orders Page
3. Contact Page
4. Shipping & Return Page
5. Peptide Purity Page

• PubChem: Alanyl-glutamyl-aspartic acid / Cartalax
• NCBI MeSH: Ala-Glu-Asp / T-31 peptide / cartalax
• FDA Gram Peptides warning letter
• Google ecommerce product data guidance
• Rank Math WooCommerce Product Schema

Ships to USA, Canada, UK & Europe

Chinese Grey Peptides supports shipping to the USA, Canada, the United Kingdom, France, Germany, Spain, Sweden, Italy, and Europe where permitted.

Shipping support may include:

• Order processing updates
• Packaging support
• Tracking information where available
• Wholesale shipment planning
• Destination-based shipping review
• Customer support during transit

Shipping times may vary by order size, carrier, destination, customs process, product status, and local requirements.

Why Choose Chinese Grey Peptides?

Chinese Grey Peptides focuses on quality, support, and clear communication. We help buyers review product details before placing an order.

Customers choose us for:

• Professional peptide supplier support
• Small and wholesale order options
• Product documentation support
• HPLC testing information where available
• Mass Spectrum documentation where available
• International shipping support
• Responsive communication
• Clear order assistance before payment

We aim to make the buying process transparent, professional, and documentation-focused.

Important Compliance Notice

Cartalax 20mg may be regulated differently depending on the country, product type, labeling, intended use, buyer type, and destination. Chinese Grey Peptides does not provide medical advice, dosing instructions, injection guidance, joint-health claims, cartilage claims, pain claims, inflammation claims, mobility claims, recovery claims, anti-aging claims, disease claims, treatment claims, or personal-use recommendations.

Customers are responsible for understanding and following all local laws, import rules, prescription requirements, compounding rules, labeling rules, sport-governing rules, and permitted-use requirements before ordering.

From its origin as a synthetic tripeptide derived from cartilage tissue research to its well‑characterised role in upregulating collagen and aggrecan gene expression, modulating MMP balance, and reducing cellular senescence markers, Cartalax (20mg) research peptide provides a precisely defined and increasingly valuable platform for advanced cartilage and connective tissue research.

Its ability to promote chondrocyte gene regulation, support collagen and proteoglycan production, reduce senescence markers, and modulate inflammatory signalling—supported by peer‑reviewed research and preclinical studies—makes it a valuable addition to any laboratory investigating osteoarthritis, cartilage biology, connective tissue repair, or the molecular mechanisms of cellular aging. By choosing high‑purity, lab‑verified Cartalax (20mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Cartalax (20mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Cartalax delivered directly to your facility in the USA, UK, or Europe.