GHK Basic (50mg) Research Peptide: An Endogenous Tripeptide for Fibroblast, ECM & Collagen Studies

If your research investigates the biology of the extracellular matrix (ECM), fibroblast dynamics, or the molecular mechanisms of tissue repair and regeneration, GHK Basic (50mg) research peptide — also known as Gly‑His‑Lys or Tripeptide‑1 — offers a naturally occurring and extensively studied synthetic tool. GHK is a tripeptide first identified in human plasma in 1973 as an activity in human albumin that caused old liver tissue to synthesise proteins like younger tissue.

As an endogenous matrikine — a signalling molecule released during ECM proteolysis — GHK functions as a high‑affinity copper(II) chelator. However, the unbound “basic” form of GHK has begun to emerge as a distinct research focus, with investigators increasingly curious about how this molecule might support processes related to structural maintenance, cellular coordination, and metabolic homeostasis independently of its copper‑bound state. This makes GHK Basic (50mg) research peptide a valuable tool for laboratories investigating fibroblast signalling, collagen synthesis, ECM remodelling, wound healing, and the molecular biology of tissue repair.

At Chinese Grey Peptides, we supply high‑purity GHK Basic (50mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to >99% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into fibroblast biology, ECM dynamics, and tissue regeneration begin with uncompromising quality.

What Is GHK Basic and How Does It Work?

To fully appreciate the research potential of GHK Basic (50mg) research peptide, it is essential to understand its biochemical identity, its role as an endogenous matrikine, and the mechanisms by which it exerts its pleiotropic effects.

The Endogenous Tripeptide

GHK Basic is a naturally occurring tripeptide with the amino acid sequence Gly‑His‑Lys (GHK). Its molecular formula is C₁₄H₂₄N₆O₄, and its molecular weight is approximately 340.4 g/mol. It has a CAS number of 49557‑75‑7.

GHK is present in human plasma, saliva, and urine. In plasma, the level of GHK is about 200 ng/mL (10⁻⁷ M) at age 20, but declines to 80 ng/mL by age 60 — a reduction that coincides with the noticeable decrease in regenerative capacity of the organism.

Mechanism of Action: Matrikine Signalling and Cellular Communication

The biological activity of GHK Basic (50mg) research peptide is mediated through several intersecting mechanisms. GHK has been shown to modulate the activity of approximately 31% of human genes, upregulating pathways involved in antioxidant defence, tissue repair, and anti‑inflammatory pathways.

Fibroblast Activation and ECM Synthesis

GHK stimulates both synthesis and breakdown of collagen and glycosaminoglycans, and modulates the activity of both metalloproteinases and their inhibitors. It stimulates collagen, dermatan sulfate, chondroitin sulfate, and the small proteoglycan decorin. GHK also restores replicative vitality to fibroblasts after radiation therapy.

High‑Affinity Copper Chelation

GHK has a very high affinity for copper and forms the chelate GHK‑Cu. The molecule’s ability to bind copper with an albumin‑like affinity means that GHK Basic readily picks up available copper and becomes GHK‑Cu. This copper‑binding property is central to many of its biological activities.

Anti‑Inflammatory and Antioxidant Effects

GHK can function as an endogenous antioxidant. It suppresses pro‑inflammatory and pro‑aging molecules like NF‑κB. GHK has been proposed as a therapeutic agent for skin inflammation.

Gene Expression Modulation

GHK has been found to up‑ and downregulate at least 4,000 human genes, essentially resetting DNA to a healthier state. It is capable of up‑ and downregulating at least 4,000 human genes, essentially resetting DNA to a healthier state.

Key Research Applications for GHK Basic (50mg)

GHK Basic (50mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Fibroblast Biology and ECM Research

As a matrikine that signals fibroblasts to synthesise ECM components, GHK Basic is a valuable tool for studying fibroblast function and ECM dynamics. Research applications include:

• Collagen Synthesis Studies: GHK stimulates the production of collagen in dermal fibroblasts. GHK stimulates collagen, dermatan sulfate, chondroitin sulfate, and the small proteoglycan, decorin.

• Fibroblast Proliferation and Migration: GHK promotes dermal fibroblast functions and restores replicative vitality to fibroblasts after radiation therapy.

• ECM Remodelling: GHK modulates the activity of both metalloproteinases and their inhibitors, regulating skin protein breakdown and potentially preventing damaged protein accumulation and excessive proteolysis.

• Gene Expression Profiling: GHK modulates approximately 31% of human genes, upregulating pathways involved in antioxidant defence.

2. Wound Healing and Tissue Repair Research

GHK has been extensively studied for its wound‑healing properties. Research has demonstrated:

• Accelerated Wound Closure: In diabetic murine models, collagen dressing loaded with GHK achieved 99.39% wound closure on day 21 compared to 69.49% for plain films.

• Diabetic Ulcers: GHK may speed up wound closure in larger ulcers and reduce the risk of wound infections. One study reported a median of 89.2% wound closure with GHK treatment.

• Enhanced Epithelialisation and Collagen Synthesis: Increased glutathione and ascorbic acid levels were found, as well as enhanced epithelialisation and increased collagen synthesis, fibroblasts, and mast cell activation in wounds.

• Increased Blood Vessel Growth: GHK‑Cu increases vascular endothelial growth factor (VEGF) production, possibly leading to growth and new blood vessel formation.

3. Fibrosis Research

GHK has demonstrated potential in modulating fibrotic pathways:

• Age‑Related Fibrosis Reversal: A 2024 study published in Aging Pathobiology and Therapeutics demonstrated that the naturally occurring peptide GHK reverses age‑related fibrosis by modulating myofibroblast function.

• Myofibroblast Apoptosis: GHK induces apoptosis in myofibroblasts, helping to resolve persistent fibrosis.

• Stemness Marker Activation: GHK promotes tissue regeneration and enhances wound healing by activating stemness markers like p63 and PCNA.

4. Antioxidant and Anti‑Inflammatory Research

GHK’s antioxidant and anti‑inflammatory properties make it a valuable tool for studying oxidative stress and inflammation:

• ROS Elimination: GHK can function as an endogenous antioxidant, possibly by diminishing ·OH and ROO·.

• Inflammation Regulation: GHK suppresses pro‑inflammatory and pro‑aging molecules like NF‑κB.

• Therapeutic Potential: GHK has been proposed as a therapeutic agent for skin inflammation, chronic obstructive pulmonary disease, and metastatic colon cancer.

5. Gene Expression and Epigenetic Research

GHK’s ability to modulate gene expression positions it as a valuable tool for studying transcriptional regulation:

• Gene Modulation: GHK modulates approximately 31% of human genes.

• Epigenetic Research: GHK is capable of up‑ and downregulating at least 4,000 human genes, essentially resetting DNA to a healthier state.

• Pathway Modulation: GHK‑Cu’s broad effects on cellular aging include upregulating pathways involved in antioxidant defence.

6. Hair Growth Research

GHK and its analogs may also have potential in follicular stimulation and growth. The copper‑containing version of GHK has been reported to increase vascular endothelial growth factor production, possibly leading to growth and new blood vessel formation.

Chemical & Physical Specifications for GHK Basic (50mg)

Accurate characterisation of GHK Basic (50mg) research peptide is essential for reproducible experimental design.

Note: The 50 mg mass refers to the weight of the peptide in its lyophilised form. GHK Basic is typically supplied as a TFA or acetate salt, which enhances aqueous solubility. The peptide is distinct from its copper‑bound form (GHK‑Cu). Because GHK binds copper with an albumin‑like affinity, copper‑free GHK converts to GHK‑Cu once copper is available.

Reconstitution & Handling Protocol for GHK Basic (50mg)

Proper reconstitution of GHK Basic (50mg) research peptide is critical for maintaining its structural integrity and biological activity. GHK Basic is a tripeptide that should be handled with standard aseptic technique. Research‑peptide vendors typically reconstitute a 50 mg vial in about 2 mL of bacteriostatic water (25 mg/mL).

Step 1 – Preparation

• Bring the sealed GHK Basic vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the GHK Basic vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 50 mg per 2 mL of diluent, yielding a 25 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted GHK Basic into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for GHK Basic (50mg)

Adjust diluent volume based on your experimental requirements. For research applications, typical doses described by vendor sources are roughly 1‑2 mg per day subcutaneously, cycled 4‑8 weeks — figures that are community/vendor convention.

Storage & Stability Guidelines for GHK Basic (50mg)

Why Choose Chinese Grey Peptides for GHK Basic (50mg)?

As an authentic licensed peptide supplier based in the USA, we supply GHK Basic (50mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed >99% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO). GHK Basic is not FDA‑approved for therapeutic use. Note: There is no human clinical trial of injectable GHK, and copper‑free GHK has never been studied by injection at all. Research and educational reference only; not a drug, not medical advice.

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: GHK Basic (50mg) Research Peptide

 What is GHK Basic (50mg) research peptide?
GHK Basic (glycyl‑L‑histidyl‑L‑lysine) is a naturally occurring tripeptide first isolated from human plasma in 1973. It is an endogenous matrikine that functions as a high‑affinity copper(II) chelator and signalling molecule involved in tissue repair, fibroblast activation, and ECM remodelling.

 What is the sequence and molecular weight of GHK Basic?
The sequence is Gly‑His‑Lys (GHK). Its molecular formula is C₁₄H₂₄N₆O₄, and its molecular weight is approximately 340.4 g/mol.

 What are the primary research applications for GHK Basic?
GHK Basic is used in fibroblast biology and ECM research (collagen synthesis, fibroblast proliferation), wound healing and tissue repair studies (diabetic ulcers, wound closure), fibrosis research (age‑related fibrosis reversal), antioxidant and anti‑inflammatory research, gene expression and epigenetic studies (4,000 gene modulation), and hair growth research.

What is the mechanism of action of GHK Basic?
GHK functions as an endogenous matrikine, signalling fibroblasts to synthesise ECM components. It binds copper with high affinity (similar to albumin), modulates metalloproteinase activity, stimulates collagen, dermatan sulfate, chondroitin sulfate, and decorin, and influences gene expression, modulating approximately 31% of human genes.

 How does GHK Basic differ from GHK‑Cu?
GHK is the free tripeptide. GHK‑Cu is the same peptide bound to copper. Because GHK binds copper with an albumin‑like affinity, copper‑free GHK converts to GHK‑Cu once copper is available. The basic unbound form is emerging as a distinct research focus.

 What is the physiological concentration of GHK in humans?
In plasma, the level of GHK is about 200 ng/mL (10⁻⁷ M) at age 20, but declines to 80 ng/mL by age 60.

What purity does your GHK Basic (50mg) meet?
Our GHK Basic is tested to >99% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute GHK Basic (50mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 25 mg/mL.

How should I store reconstituted GHK Basic?
Reconstituted GHK Basic should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

 Is GHK Basic research peptide legal to purchase in the USA?
Yes. GHK Basic is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: There is no human clinical trial of injectable GHK, and copper‑free GHK has never been studied by injection at all.

 Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

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Important Compliance Notice

GHK Basic (50mg) may be regulated differently depending on the country, product type, labeling, intended use, buyer type, and destination. Chinese Grey Peptides does not provide medical advice, dosing instructions, injection guidance, IV guidance, anti-aging claims, energy claims, longevity claims, detox claims, mitochondrial claims, disease claims, treatment claims, or personal-use recommendations.

Customers are responsible for understanding and following all local laws, import rules, supplement rules, prescription requirements, compounding rules, labeling rules, advertising rules, and permitted-use requirements before ordering.

From its discovery as an endogenous matrikine in human plasma to its well‑characterised role in fibroblast activation, collagen synthesis, and ECM remodelling, GHK Basic (50mg) research peptide provides a precisely defined and extensively validated platform for advanced tissue repair and regenerative research.

Its ability to stimulate collagen synthesis, modulate MMP activity, influence the expression of approximately 4,000 human genes, and promote wound healing with up to 99% closure in diabetic models—supported by over five decades of peer‑reviewed research published in PubMed, PMC, ScienceDirect, and other authoritative journals—makes it a valuable addition to any laboratory investigating fibroblast biology, ECM dynamics, wound healing, or the molecular mechanisms of tissue regeneration. By choosing high‑purity, lab‑verified GHK Basic (50mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with GHK Basic (50mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade GHK Basic delivered directly to your facility in the USA, UK, or Europe.GHK Basic (50mg), GHK Basic (50mg)