Tripeptide-29 (200mg) Research Peptide: A Gly-Pro-Hyp Collagen Building Block for Dermal & Extracellular Matrix Studies

If your research investigates extracellular matrix (ECM) biology, collagen synthesis, or the molecular mechanisms underlying dermal repair and photoaging, Tripeptide-29 (200mg) research peptide — also known as Gly‑Pro‑Hyp or Collagen Tripeptide — offers a precisely defined synthetic tool that replicates the fundamental repeating unit of native collagen. This tripeptide, consisting of glycine, proline, and hydroxyproline (Gly‑Pro‑Hyp), represents one of the most common motifs found in Type I collagen, the most abundant protein in mammals.

As a synthetic analogue of a collagen building block, Tripeptide-29 serves as a versatile framework for studying collagen fibrillogenesis, fibroblast signalling, ECM remodelling, and the broader biological roles of collagen‑derived peptides. This makes Tripeptide-29 (200mg) research peptide a valuable tool for laboratories investigating connective tissue biology, dermal research, wound healing, and metabolic regulation.

At Chinese Grey Peptides, we supply high‑purity Tripeptide-29 (200mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into collagen biology, fibroblast signalling, and ECM dynamics begin with uncompromising quality.

What Is Tripeptide-29 and How Does It Work?

To fully appreciate the research potential of Tripeptide-29 (200mg) research peptide, it is essential to understand its biochemical identity, its role as a collagen building block, and the mechanisms by which it exerts its pleiotropic effects.

The Gly‑Pro‑Hyp Tripeptide

Tripeptide‑29 is a synthetic tripeptide with the amino acid sequence Gly‑Pro‑Hyp (glycine‑proline‑hydroxyproline). Its molecular formula is C₁₂H₁₉N₃O₅, with a molecular weight of approximately 285.3 g/mol. It has a CAS number of 2239‑67‑0.

Collagen is a long‑chain polymer made of short monomeric repeats that generally follow the pattern Gly‑Pro‑X or Gly‑X‑Hyp. Tripeptide‑29, with its Gly‑Pro‑Hyp sequence, is a perfect synthetic analogue of common collagen building blocks. It is also referred to as Glycylprolylhydroxyproline or Collagen Tripeptide.

Mechanism of Action: Collagen Stability, Fibroblast Signalling & Enzymatic Modulation

The biological activity of Tripeptide-29 (200mg) research peptide is mediated through several intersecting mechanisms:

Collagen Stability and Structural Integrity

As a fundamental building block of collagen, Tripeptide‑29 may contribute to the stability of Type I collagen molecules. The hydroxyproline (Hyp) residue in Tripeptide‑29 is thought to contribute to the formation of favourable interatomic interactions that enhance the stability of collagen and collagen microfibrils. Collagen is a major component of the extracellular matrix and is considered responsible for providing structure and support to cellular complexes.

Fibroblast Activation and Collagen Synthesis

Tripeptide‑29 appears to interact with skin cells to support extracellular matrix dynamics. By mimicking specific sequences found in native collagen, it can modulate cellular signalling pathways related to collagen synthesis and turnover. It may activate signalling pathways in skin fibroblasts, such as the TGF‑β pathway, to upregulate collagen I (COL1A1) gene expression. Research indicates that Tripeptide‑29 may stimulate collagen synthesis and promote the formation of collagen fibres.

DPP‑IV Inhibition and Metabolic Regulation

Emerging research suggests that Tripeptide‑29 may serve as a peptide inhibitor of dipeptidyl peptidase‑IV (DPP‑IV), an enzyme involved in the cleavage of dipeptides and tripeptides. Specifically, Tripeptide‑29 appears to inhibit the hydrolysis of the Pro‑Hyp bond, acting as a competitive inhibitor. Notably, Tripeptide‑29 is suggested to resist hydrolysis by DPP‑IV, indicating it may retain stability and functionality in the presence of this enzyme. DPP‑IV cleaves GLP‑1 and GIP, hormones involved in glucose regulation, so inhibiting DPP‑IV may potentially elevate levels of active GLP‑1 and GIP.

Antioxidant and Anti‑Inflammatory Effects

Research studies have hypothesised Tripeptide‑29’s potential as an antioxidant, helping to protect cells from oxidative stress and damage. It may also exhibit anti‑inflammatory and anti‑fibrotic characteristics.

Key Research Applications for Tripeptide‑29 (200mg)

Tripeptide-29 (200mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Collagen Synthesis & ECM Biology Research

As a fundamental building block of collagen, Tripeptide‑29 is an essential tool for studying ECM biology and collagen dynamics. Research applications include:

• Collagen Fibrillogenesis: Investigating how the Gly‑Pro‑Hyp motif contributes to the assembly of the triple‑helix structure characteristic of collagen fibres.

• Extracellular Matrix Remodelling: Studying how collagen‑derived peptides influence ECM organisation and stability.

• Connective Tissue Biology: Exploring the role of collagen in tendons, ligaments, skin, vascular walls, bone, and cartilage.

2. Fibroblast & Dermal Research

Tripeptide‑29’s ability to interact with fibroblasts and stimulate collagen synthesis positions it as a valuable tool for dermal research. Research applications include:

• Fibroblast Signalling: Investigating how Tripeptide‑29 modulates cellular signalling pathways related to collagen synthesis and turnover.

• Photoaging and UVB‑Induced Damage: Studying how Tripeptide‑29 attenuates inflammation and reduces MMP‑1 and MMP‑3 levels in response to UVB‑induced skin aging.

• Anti‑Wrinkle Research: Exploring the potential of Tripeptide‑29 to improve skin hydration, elasticity, and wrinkle appearance.

3. DPP‑IV Inhibition & Metabolic Research

Tripeptide‑29’s role as a competitive inhibitor of DPP‑IV makes it a valuable tool for metabolic research. Research applications include:

• Glucose Regulation: Investigating how DPP‑IV inhibition may influence GLP‑1 and GIP levels.

• Metabolic Pathway Studies: Exploring the broader implications of DPP‑IV inhibition in energy metabolism and glucose homeostasis.

4. Platelet Activation & Haemostasis Research

Collagen‑related peptides containing the Gly‑Pro‑Hyp motif are hypothesised to play a role in platelet activation and aggregation via interactions with glycoprotein VI (GPVI) receptors. Research applications include:

• Platelet Aggregation Studies: Investigating how Tripeptide‑29 acts as a partial agonist of the collagen receptor GPVI.

• Haemostatic Mechanisms: Exploring the role of collagen‑derived peptides in the early formation of blood clots and tissue repair.

5. Antioxidant & Anti‑Inflammatory Research

Tripeptide‑29’s potential antioxidant and anti‑inflammatory properties make it a valuable tool for studying oxidative stress and inflammation. Research applications include:

• Oxidative Stress: Investigating how Tripeptide‑29 reduces oxidative stress and damage in dermal fibroblasts.

• Glycation Inhibition: Studying how Tripeptide‑29 may reduce the build‑up of advanced glycation end products (AGEs) and prevent the production of denatured collagen.

Chemical & Physical Specifications for Tripeptide-29 (200mg)

Accurate characterisation of Tripeptide-29 (200mg) research peptide is essential for reproducible experimental design.

Note: The 200 mg mass refers to the weight of the peptide in its lyophilised form. Tripeptide‑29 is typically supplied as a TFA or acetate salt. The palmitoylated form (Pal‑Gly‑Pro‑Hyp) is commonly used in cosmetic formulations, but this product is the free peptide for research use only (RUO).

Reconstitution & Handling Protocol for Tripeptide-29 (200mg)

Proper reconstitution of Tripeptide-29 (200mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Tripeptide‑29 vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Tripeptide‑29 vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 200 mg per 20 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Tripeptide‑29 into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for Tripeptide-29 (200mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for Tripeptide-29 (200mg)

Why Choose Chinese Grey Peptides for Tripeptide-29 (200mg)?

As an authentic licensed peptide supplier based in the USA, we supply Tripeptide-29 (200mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Tripeptide-29 (200mg) Research Peptide

What is Tripeptide-29 (200mg) research peptide?
Tripeptide‑29 is a synthetic tripeptide with the sequence Gly‑Pro‑Hyp (glycine‑proline‑hydroxyproline). It is a synthetic mimic of a naturally occurring collagen building block and is used in research to study collagen synthesis, ECM biology, fibroblast signalling, and dermal repair.

 What is the sequence and molecular weight of Tripeptide‑29?
The sequence is Gly‑Pro‑Hyp. Its molecular formula is C₁₂H₁₉N₃O₅, and its molecular weight is approximately 285.3 g/mol.

 What are the primary research applications for Tripeptide‑29?
Tripeptide‑29 is used in collagen synthesis and ECM biology research, fibroblast and dermal research (photoaging, anti‑wrinkle), DPP‑IV inhibition and metabolic research, platelet activation and haemostasis studies, and antioxidant and anti‑inflammatory research.

What is the mechanism of action of Tripeptide‑29?
Tripeptide‑29 acts as a fundamental building block of collagen, contributing to collagen stability and fibrillogenesis. It may activate fibroblast signalling pathways (such as TGF‑β) to upregulate collagen I gene expression. It also acts as a competitive inhibitor of DPP‑IV and may interact with GPVI receptors on platelets.

 What are the antioxidant and anti‑inflammatory properties of Tripeptide‑29?
Research suggests Tripeptide‑29 may act as an antioxidant, protecting cells from oxidative stress. It also reduces MMP‑1 and MMP‑3 levels, attenuates inflammation, and may exhibit anti‑fibrotic characteristics.

 What purity does your Tripeptide-29 (200mg) meet?
Our Tripeptide‑29 is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Tripeptide-29 (200mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

 How should I store reconstituted Tripeptide‑29?
Reconstituted Tripeptide‑29 should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

 Is Tripeptide-29 research peptide legal to purchase in the USA?
Yes. Tripeptide‑29 is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims.

 Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

Ships to USA, Canada, UK & Europe

Chinese Grey Peptides supports shipping to the USA, Canada, the United Kingdom, France, Germany, Spain, Sweden, Italy, and Europe where permitted.

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Shipping times may vary by order size, carrier, destination, customs process, product status, and local requirements.

Why Choose Chinese Grey Peptides?

Chinese Grey Peptides focuses on quality, support, and clear communication. We help buyers review product details before placing an order.

Customers choose us for:

• Professional peptide supplier support
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• PubChem Glycyl-prolyl-hydroxyproline
• PubChem Gly-Pro-Hyp
• FDA Cosmetics Labeling Claims
• FDA: Is It a Cosmetic, a Drug, or Both?
• Google Product Structured Data
• Rank Math WooCommerce Product Schema

From its identity as a fundamental collagen building block to its well‑characterised roles in fibroblast signalling, DPP‑IV inhibition, and antioxidant protection, Tripeptide-29 (200mg) research peptide provides a precisely defined and extensively validated platform for advanced ECM and dermal research. Its ability to mimic the Gly‑Pro‑Hyp motif characteristic of Type I collagen, stimulate collagen synthesis, and modulate enzymatic and signalling pathways—supported by peer‑reviewed research published in PubMed, PMC, and other authoritative journals—makes it a valuable addition to any laboratory investigating collagen biology, fibroblast function, dermal repair, or metabolic regulation. By choosing high‑purity, lab‑verified Tripeptide-29 (200mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Tripeptide-29 (200mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Tripeptide‑29 delivered directly to your facility in the USA, UK, or Europe.