Cardiogen (20mg) Research Peptide: An AEDR Tetrapeptide for Cardiac & Cellular Repair Studies

If your research investigates cardiac tissue regeneration, fibroblast regulation, or the molecular mechanisms of cellular repair and apoptosis, Cardiogen (20mg) research peptide — also known as the AEDR tetrapeptide (Ala‑Glu‑Asp‑Arg) — offers a precisely defined synthetic tool. Belonging to the Khavinson family of peptide bioregulators, Cardiogen is a short, biologically active tetrapeptide developed from heart tissue‑derived polypeptide complexes.

It is classified as a cardiovascular bioregulator peptide and is investigated in preclinical settings for its effects on cardiac tissues, fibroblasts, cellular repair mechanisms and apoptosis regulation. Research suggests that Cardiogen may stimulate cardiac cell proliferation, inhibit fibroblast-driven scar formation, and reduce apoptosis by suppressing p53 protein expression. This makes Cardiogen (20mg) research peptide a valuable tool for laboratories investigating cardiovascular biology, tissue regeneration, fibroblast signalling, and the molecular pathways underlying heart function and repair.

At Chinese Grey Peptides, we supply high‑purity Cardiogen (20mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into cardiac biology, fibroblast regulation, and cellular repair begin with uncompromising quality.

What Is Cardiogen and How Does It Work?

To fully appreciate the research potential of Cardiogen (20mg) research peptide, it is essential to understand its biochemical identity and the mechanisms by which it exerts its effects.

The AEDR Tetrapeptide

Cardiogen is a synthetic tetrapeptide consisting of four amino acids: alanine, glutamic acid, aspartic acid, and arginine — the sequence Ala‑Glu‑Asp‑Arg (AEDR) . Its molecular formula is C₁₈H₃₁N₇O₉, with a molecular weight of approximately 489.5 Da. It has a PubChem CID of 11583989. The peptide belongs to the Khavinson family of short peptide bioregulators — molecules hypothesised to interact with chromatin and DNA to modulate gene expression in a tissue‑specific manner. It was developed by Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology.

Mechanism of Action: Cardiac Repair, Fibroblast Regulation & Gene Modulation

The biological activity of Cardiogen (20mg) research peptide is hypothesised to involve several intersecting mechanisms:

Cardiomyocyte Proliferation and Cardiac Repair

Research suggests Cardiogen stimulates cardiac cell proliferation and promotes heart muscle regeneration. In heart tissue cultures from rats aged 3 months (young) and 24 months (old), Cardiogen uniquely activated cardiomyocyte proliferation while suppressing apoptosis markers like p53 protein. Notably, Cardiogen’s performance remained robust across both age groups, maintaining its efficacy even in aged tissue where natural regenerative capacity diminishes. An immunohistochemical study demonstrated a decrease of p53 protein expression by Cardiogen action.

Fibroblast Regulation and Scar Formation Inhibition

Cardiogen targets fibroblasts — key cells in tissue repair — and is hypothesised to inhibit fibroblast-driven scar formation. It may primarily target fibroblasts, which play a crucial role in the formation of scars and tissue regeneration. This dual action — promoting cardiomyocyte proliferation while reducing fibroblast activity — may contribute to reduced scar formation and improved cardiac remodelling. Cardiogen treatment has been shown to reduce collagen I and III expression in aging rat myocardium, leading to decreased fibrotic remodelling.

Apoptosis Regulation and p53 Suppression

Cardiogen reduces apoptosis by suppressing p53 protein expression. The tetrapeptide demonstrated a great stimulating effect on proliferation in myocard tissue cultures from both young and old rats. Researchers consider the peptide to be an apoptotic reductant in cardiac cells via possibly decreasing p53 expression.

DNA Interaction and Gene Expression Modulation

Cardiogen has been shown to modulate DNA interactions. It does not bind single‑stranded deoxyribooligonucleotides, slightly quenches fluorescence of some double‑stranded deoxyribooligonucleotides, and strongly quenches fluorescence of methylated and unmethylated λ phage DNA‑ethidium bromide complexes. Its ability to inhibit DNA hydrolysis indicates that it can control endonuclease DNA hydrolysis, and this effect may be mediated through interaction with the enzyme rather than direct binding to DNA.

Key Research Applications for Cardiogen (20mg)

Cardiogen (20mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Cardiac & Cardiovascular Research

As a cardiovascular bioregulator peptide, Cardiogen is a valuable tool for studying heart function and repair. Research applications include:

• Cardiomyocyte Proliferation: Investigating how Cardiogen stimulates cardiac cell proliferation and regeneration.

• Myocardial Injury Models: Studying Cardiogen’s effects in models of myocardial infarction, heart failure, and ischaemia–reperfusion injury.

• Cardiac Remodelling: Exploring how Cardiogen reduces scar formation and improves cardiac remodelling.

• Age‑Related Cardiac Dysfunction: Investigating Cardiogen’s efficacy in aged tissue where natural regenerative capacity diminishes.

2. Fibroblast & Extracellular Matrix Research

As a peptide that targets fibroblasts, Cardiogen is a valuable tool for studying fibroblast biology and ECM dynamics. Research applications include:

• Fibroblast Regulation: Investigating how Cardiogen influences fibroblast activity and scar formation.

• Tissue Repair and Scar Formation: Studying Cardiogen’s effects on fibroblast-driven tissue repair and scar formation.

• Collagen Synthesis: Investigating how Cardiogen reduces collagen I and III expression in aging myocardium.

3. Apoptosis & Cell Signalling Research

Cardiogen’s effects on apoptosis and gene expression make it a valuable tool for studying cell signalling and survival pathways:

• p53 Pathway Studies: Investigating how Cardiogen suppresses p53 protein expression and reduces apoptosis.

• Apoptosis Regulation: Exploring Cardiogen’s role as an apoptotic reductant in cardiac cells.

• Gene Expression Modulation: Studying how Cardiogen regulates gene expression and cellular proliferation.

4. Oncology Research

Cardiogen has been investigated for its effects on tumour cells:

• Tumour Apoptosis: Cardiogen may promote tumour cell apoptosis in sarcoma models.

• Tumour Growth Inhibition: In M-1 sarcoma models, Cardiogen induced hemorrhagic necrosis and stimulated tumour cell apoptosis in a dose‑dependent manner. The cytostatic effect was not direct, but rather realised through the vascular network of the tumour.

5. Cellular Protection & Regeneration Research

Cardiogen supports the synthesis of cytoskeletal proteins including actin, vimentin, and tubulin, while increasing nuclear matrix proteins lamin A and C by up to 2.5‑fold. Research applications include:

• Cytoskeletal Integrity: Studying how Cardiogen supports the structural integrity of cardiac cells.

• Nuclear Matrix Research: Investigating the role of lamin A and C in cardiac cell function and repair.

• Regenerative Signalling: Exploring how Cardiogen promotes progenitor‑like cell proliferation within cardiac tissues.

Chemical & Physical Specifications for Cardiogen (20mg)

Accurate characterisation of Cardiogen (20mg) research peptide is essential for reproducible experimental design.

Note: The 20 mg mass refers to the weight of the peptide in its lyophilised form. Cardiogen is a Khavinson tetrapeptide bioregulator. The peptide is for research use only (RUO). Not for human consumption. Cardiogen is not FDA‑approved for therapeutic use.

Reconstitution & Handling Protocol for Cardiogen (20mg)

Proper reconstitution of Cardiogen (20mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Cardiogen vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Cardiogen vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 20 mg per 2 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Cardiogen into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for Cardiogen (20mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for Cardiogen (20mg)

Why Choose Chinese Grey Peptides for Cardiogen (20mg)?

As an authentic licensed peptide supplier based in the USA, we supply Cardiogen (20mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO). Cardiogen is not FDA‑approved for therapeutic use.

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Cardiogen (20mg) Research Peptide

 What is Cardiogen (20mg) research peptide?
Cardiogen is a synthetic tetrapeptide with the sequence Ala‑Glu‑Asp‑Arg (AEDR). It belongs to the Khavinson family of peptide bioregulators and is classified as a cardiovascular bioregulator peptide, investigated in preclinical settings for its effects on cardiac tissues, fibroblasts, cellular repair mechanisms and apoptosis regulation.

 What is the sequence and molecular weight of Cardiogen?
The sequence is Ala‑Glu‑Asp‑Arg (AEDR). Its molecular formula is C₁₈H₃₁N₇O₉, and its molecular weight is approximately 489.5 g/mol.

What are the primary research applications for Cardiogen?
Cardiogen is used in cardiac and cardiovascular research (cardiomyocyte proliferation, myocardial injury models, cardiac remodelling), fibroblast and extracellular matrix research, apoptosis and cell signalling research (p53 pathway studies), oncology research (tumour apoptosis), and cellular protection and regeneration research.

What is the mechanism of action of Cardiogen?
Cardiogen stimulates cardiac cell proliferation, inhibits fibroblast-driven scar formation, and reduces apoptosis by suppressing p53 protein expression. It also modulates DNA interactions and supports the synthesis of cytoskeletal proteins including actin, vimentin, and tubulin.

What evidence supports Cardiogen’s effects on aged cardiac tissue?
In heart tissue cultures from rats aged 3 months and 24 months, Cardiogen demonstrated a great stimulating effect on proliferation both in tissues from young and old rats, maintaining its efficacy even in aged tissue.

 What purity does your Cardiogen (20mg) meet?
Our Cardiogen is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

 How should I reconstitute Cardiogen (20mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

How should I store reconstituted Cardiogen?
Reconstituted Cardiogen should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Cardiogen research peptide legal to purchase in the USA?
Yes. Cardiogen is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: Cardiogen is not FDA‑approved for therapeutic use and has not been evaluated by any Western regulatory agency.

Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

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• PubChem: H-Ala-Glu-Asp-Arg-OH / AEDR
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Important Compliance Notice

Cardiogen 20mg may be regulated differently depending on the country, product type, labeling, intended use, buyer type, and destination. Chinese Grey Peptides does not provide medical advice, dosing instructions, injection guidance, cardiac claims, heart-health claims, cardiovascular claims, blood-flow claims, anti-aging claims, recovery claims, disease claims, treatment claims, or personal-use recommendations.

Customers are responsible for understanding and following all local laws, import rules, prescription requirements, compounding rules, labeling rules, sport-governing rules, and permitted-use requirements before ordering.

From its origin as a tetrapeptide developed from heart tissue‑derived polypeptide complexes to its well‑characterised role in stimulating cardiomyocyte proliferation, inhibiting fibroblast-driven scar formation, and modulating p53‑mediated apoptosis, Cardiogen (20mg) research peptide provides a precisely defined and increasingly valuable platform for advanced cardiovascular and cellular research. Its ability to promote cardiac repair, regulate fibroblast activity, and modulate gene expression — supported by peer‑reviewed research published in PubMed and other authoritative journals — makes it a valuable addition to any laboratory investigating cardiac biology, tissue regeneration, fibroblast signalling, or the molecular mechanisms of cellular repair. By choosing high‑purity, lab‑verified Cardiogen (20mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Cardiogen (20mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Cardiogen delivered directly to your facility in the USA, UK, or Europe.