Nonapeptide-1 (200mg) Research Peptide: A Selective MC1R Antagonist for Melanogenesis & Pigmentation Studies

If your research investigates melanocortin receptor signalling, melanogenesis pathways, or the molecular mechanisms of skin pigmentation and adrenal steroidogenesis, Nonapeptide-1 (200mg) research peptide — also known as Melanostatine‑5 — offers a precisely defined synthetic tool with potent and selective antagonist activity. This synthetic nonapeptide (Met‑Pro‑D‑Phe‑Arg‑D‑Trp‑Phe‑Lys‑Pro‑Val‑NH₂) acts as a competitive antagonist of the melanocortin 1 receptor (MC1R), effectively blocking α‑melanocyte‑stimulating hormone (α‑MSH) binding to its target receptor.

With a binding affinity (Ki) of 40 nM and an IC₅₀ of 2.5 nM for inhibiting intracellular cAMP accumulation induced by α‑MSH in melanocytes, Nonapeptide‑1 provides researchers with a highly selective tool for studying the molecular regulation of melanin synthesis, adrenal steroid production, and the broader implications of MC1R signalling in skin biology and cancer research. This makes Nonapeptide-1 (200mg) research peptide a valuable tool for laboratories investigating pigmentation disorders, endocrine regulation, and the molecular mechanisms underlying melanocyte function.

At Chinese Grey Peptides, we supply high‑purity Nonapeptide-1 (200mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into melanogenesis, MC1R signalling, and pigmentary biology begin with uncompromising quality.

What Is Nonapeptide‑1 and How Does It Work?

To fully appreciate the research potential of Nonapeptide-1 (200mg) research peptide, it is essential to understand its biochemical identity, its role as a selective MC1R antagonist, and the mechanisms by which it exerts its effects.

The Synthetic Nonapeptide

Nonapeptide‑1 is a synthetic linear nonapeptide with the amino acid sequence Met‑Pro‑D‑Phe‑Arg‑D‑Trp‑Phe‑Lys‑Pro‑Val‑NH₂. Its molecular formula is C₆₁H₈₇N₁₅O₉S, with a molecular weight of approximately 1,206.5 g/mol. It has a CAS number of 158563‑45‑2. The presence of D‑amino acids (D‑Phe and D‑Trp) in the sequence enhances the peptide’s stability and resistance to enzymatic degradation, making it a robust research tool. Structural analysis has revealed that residues at positions 5‑6, 7‑9, and 10 are critical for its antagonistic activity, with D‑Trp5 and Phe6 playing pivotal roles in receptor inactivation.

Nonapeptide‑1 originated in the early 1990s as melanostatine, an antibiotic metabolite isolated from Streptomyces clavifer strain N924‑2. Today, it is recognised as a selective antagonist of the melanocortin 1 receptor (MC1R), a G‑protein‑coupled receptor predominantly expressed on melanocytes.

Mechanism of Action: Selective MC1R Antagonism

The biological activity of Nonapeptide-1 (200mg) research peptide is mediated through its high‑affinity, selective binding to the melanocortin 1 receptor (MC1R):

• High‑Affinity Receptor Binding: Nonapeptide‑1 binds to MC1R with a binding affinity (Ki) of 40 nM. It is selective for MC1R over MC3R, MC4R, and MC5R, with Ki values of 470 nM, 1,340 nM, and 2,400 nM, respectively.

• Competitive α‑MSH Antagonism: The peptide functions as a competitive antagonist of α‑melanocyte‑stimulating hormone (α‑MSH). By binding to MC1R and blocking the receptor site, it prevents α‑MSH from activating the receptor, thereby inhibiting the downstream signalling cascade that drives melanogenesis.

• Inhibition of cAMP Accumulation: Receptor activation by α‑MSH normally triggers an increase in intracellular cyclic AMP (cAMP). Nonapeptide‑1 inhibits α‑MSH‑induced cAMP accumulation with an IC₅₀ of 2.5 nM.

• Inhibition of Melanosome Dispersion: The peptide also inhibits α‑MSH‑induced melanosome dispersion in melanocytes with an IC₅₀ of 11 nM.

• Downregulation of Melanin Synthesis: By blocking MC1R signalling, Nonapeptide‑1 suppresses the downstream activation of melanogenic enzymes, leading to a significant reduction in melanin synthesis.

Key Research Applications for Nonapeptide-1 (200mg)

Nonapeptide‑1 (200mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Melanogenesis & Pigmentation Research

As a potent MC1R antagonist that inhibits melanin synthesis, Nonapeptide‑1 is a valuable tool for studying the molecular regulation of skin pigmentation. Research applications include:

• Melanocyte Signalling Studies: Investigating the α‑MSH‑MC1R‑cAMP signalling cascade and its role in melanocyte biology.

• Hyperpigmentation Models: Exploring the molecular mechanisms underlying hyperpigmentation disorders such as melasma, age spots, and post‑inflammatory hyperpigmentation.

• Melanin Synthesis Inhibition: Nonapeptide‑1, at a concentration of 200 µg/mL, is capable of fully inhibiting the synthesis of melanin in fungi.

• Comparative Melanogenesis Studies: Understanding how different melanocortin receptor ligands influence pigmentation pathways.

2. MC1R Receptor Pharmacology & Signalling Research

As a selective MC1R antagonist, Nonapeptide‑1 is a valuable tool for receptor pharmacology studies. Research applications include:

• Receptor Binding Assays: Characterising the binding affinity, kinetics, and selectivity of MC1R ligands.

• Signal Transduction Studies: Dissecting the G‑protein‑coupled signalling pathways activated by MC1R engagement.

• Structure‑Activity Relationship (SAR) Studies: Investigating how modifications to the Nonapeptide‑1 sequence affect receptor binding and activity.

• Receptor Localisation: Using labelled Nonapeptide‑1 for live‑cell imaging of MC1R.

3. Adrenal Steroidogenesis Research

Beyond its role in pigmentation, Nonapeptide‑1 has been studied for its effects on steroid production in the adrenal gland. Research applications include:

• Adrenal Function Studies: Investigating the role of MC1R and related melanocortin receptors in adrenal steroidogenesis.

• Steroid Regulation: Exploring the regulation of steroid production and its modulation by peptide antagonists.

• Endocrine Pathway Mapping: Understanding the interplay between melanocortin receptor signalling and adrenal hormone production.

4. Skin Cancer Research

Nonapeptide‑1 is also relevant to skin cancer research. Research applications include:

• Melanoma Biology: Investigating the role of MC1R signalling in melanoma development and progression.

• Pigmentary Pathways: Exploring the relationship between melanogenesis pathways and skin cancer risk.

• Therapeutic Target Validation: Using Nonapeptide‑1 to validate MC1R as a potential target for skin cancer research.

5. Formulation & Nanotechnology Research

Nonapeptide‑1 has been investigated in advanced delivery systems. Research applications include:

• Peptide Conjugation Studies: Exploring Nonapeptide‑1‑conjugated nanoparticles for targeted delivery.

• Stability Studies: Investigating the stability and bioavailability of Nonapeptide‑1 in various formulation contexts.

• Transdermal Delivery: Studying the skin permeability of Nonapeptide‑1 and its formulation requirements.

Chemical & Physical Specifications

Accurate characterisation of Nonapeptide‑1 (200mg) research peptide is essential for reproducible experimental design.

Note: The 200 mg mass refers to the weight of the peptide in its lyophilised form. Nonapeptide‑1 is typically supplied as an acetate salt, which enhances aqueous solubility. The peptide is for research use only (RUO). Not for human consumption. Nonapeptide‑1 is not FDA‑approved for therapeutic use.

Reconstitution & Handling Protocol for Nonapeptide‑1 (200mg)

Proper reconstitution of Nonapeptide‑1 (200mg) research peptide is critical for maintaining its structural integrity and biological activity. The peptide is soluble in DMSO and appropriate buffers.

Step 1 – Preparation

• Bring the sealed Nonapeptide‑1 vial and your chosen solvent to room temperature.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Nonapeptide‑1 vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of solvent into a sterile syringe. A common starting concentration for research applications is 200 mg per 20 mL of DMSO or appropriate buffer, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the solvent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Nonapeptide‑1 into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for Nonapeptide-1 (200mg)

Adjust solvent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for Nonapeptide-1 (200mg)

Why Choose Chinese Grey Peptides for Nonapeptide‑1 (200mg)?

As an authentic licensed peptide supplier based in the USA, we supply Nonapeptide-1 (200mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO). Nonapeptide‑1 is not FDA‑approved for therapeutic use.

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Nonapeptide-1 (200mg) Research Peptide

What is Nonapeptide-1 (200mg) research peptide?
Nonapeptide‑1 (Melanostatine‑5) is a synthetic nonapeptide (Met‑Pro‑D‑Phe‑Arg‑D‑Trp‑Phe‑Lys‑Pro‑Val‑NH₂) that acts as a selective antagonist of the melanocortin 1 receptor (MC1R). It competitively blocks α‑MSH binding, inhibiting cAMP accumulation, melanosome dispersion, and melanin synthesis.

 What is the sequence and molecular weight of Nonapeptide‑1?
The sequence is Met‑Pro‑D‑Phe‑Arg‑D‑Trp‑Phe‑Lys‑Pro‑Val‑NH₂. Its molecular formula is C₆₁H₈₇N₁₅O₉S, and its molecular weight is approximately 1,206.5 g/mol.

 What are the primary research applications for Nonapeptide‑1?
Nonapeptide‑1 is used in melanogenesis and pigmentation research (melanocyte signalling, hyperpigmentation models), MC1R receptor pharmacology and signalling studies, adrenal steroidogenesis research, skin cancer research, and formulation and nanotechnology research.

 What is the mechanism of action of Nonapeptide‑1?
Nonapeptide‑1 acts as a competitive antagonist at the MC1R (Ki = 40 nM), blocking α‑MSH from activating the receptor. It inhibits α‑MSH‑induced cAMP accumulation (IC₅₀ = 2.5 nM) and melanosome dispersion (IC₅₀ = 11 nM), and downregulates melanin synthesis.

 What is the selectivity of Nonapeptide‑1 for MC1R?
Nonapeptide‑1 is selective for MC1R over MC3R, MC4R, and MC5R, with Ki values of 470 nM, 1,340 nM, and 2,400 nM, respectively.

 What purity does your Nonapeptide‑1 (200mg) meet?
Our Nonapeptide‑1 is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

 How should I reconstitute Nonapeptide-1 (200mg)?
Reconstitute with DMSO or appropriate buffer. Allow the vial to reach room temperature, then slowly inject the solvent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

 How should I store reconstituted Nonapeptide‑1?
Reconstituted Nonapeptide‑1 should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

 Is Nonapeptide‑1 research peptide legal to purchase in the USA?
Yes. Nonapeptide‑1 is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: Nonapeptide‑1 is not FDA‑approved for therapeutic use.

 Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

Nonapeptide-1 (200mg)

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Nonapeptide-1 (200mg)

Important Compliance Notice

Nonapeptide-1 (200mg) may be regulated differently depending on the country, product type, labeling, intended use, buyer type, and destination. Chinese Grey Peptides does not provide medical advice, dosing instructions, injection guidance, skin-lightening guarantees, pigmentation-treatment claims, melanin-inhibition claims, anti-aging claims, disease claims, treatment claims, cosmetic formulation instructions, or personal-use recommendations.

Customers are responsible for understanding and following all local laws, import rules, cosmetic ingredient requirements, prescription requirements, compounding rules, labeling rules, advertising rules, and permitted-use requirements before ordering.

Nonapeptide-1 (200mg)

From its discovery as a melanocortin receptor antagonist to its well‑characterised role as a selective MC1R antagonist with potent inhibitory activity, Nonapeptide-1 (200mg) research peptide provides a precisely defined and increasingly valuable platform for advanced melanogenesis and receptor pharmacology research.

Its ability to inhibit α‑MSH‑induced cAMP accumulation (IC₅₀ = 2.5 nM) and melanosome dispersion (IC₅₀ = 11 nM) with high selectivity (Ki = 40 nM for MC1R)—supported by peer‑reviewed research published in PubMed and other authoritative sources—makes it a valuable addition to any laboratory investigating melanogenesis, MC1R signalling, adrenal steroidogenesis, or skin cancer biology. By choosing high‑purity, lab‑verified Nonapeptide-1 (200mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Nonapeptide-1 (200mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Nonapeptide‑1 delivered directly to your facility in the USA, UK, or Europe.Nonapeptide-1 (200mg)