Bronchogen (20mg) Research Peptide: An AEDL Tetrapeptide for Respiratory & Epigenetic Studies

If your research investigates pulmonary epithelial biology, DNA stability, or the epigenetic regulation of gene expression, Bronchogen (20mg) research peptide — also known as the tetrapeptide AEDL (Ala‑Glu‑Asp‑Leu) or T‑33 — offers a precisely defined synthetic tool with a growing body of scientific literature. Bronchogen belongs to the Khavinson family of peptide bioregulators, a class of short peptides hypothesised to interact directly with DNA and chromatin to modulate gene expression in a tissue‑specific manner.

Developed based on polypeptide sequences from bronchial mucosa, Bronchogen has been shown to regulate the synthesis of key proteins in bronchial epithelial cells, including Ki67, Mcl‑1, p53, CD79, and NOS‑3. It also activates the expression of differentiation factors such as Nkx2.1, SCGB1A1, SCGB3A2, FoxA1, and FoxA2, as well as MUC4, MUC5AC, and SftpA — markers of functional activity in bronchial epithelial cells. This makes Bronchogen (20mg) research peptide a valuable tool for laboratories investigating respiratory biology, epigenetics, inflammation, and the molecular mechanisms of tissue repair.

At Chinese Grey Peptides, we supply high‑purity Bronchogen (20mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into respiratory biology, gene regulation, and tissue repair begin with uncompromising quality.

What Is Bronchogen and How Does It Work?

To fully appreciate the research potential of Bronchogen (20mg) research peptide, it is essential to understand its biochemical identity and the mechanisms by which it exerts its effects.

The AEDL Tetrapeptide

Bronchogen is a synthetic tetrapeptide consisting of four amino acids: alanine, glutamic acid, aspartic acid, and leucine — the sequence Ala‑Glu‑Asp‑Leu (AEDL). Its molecular formula is C₁₈H₃₀N₄O₉, and its molecular weight is approximately 446.45 g/mol. It has a molecular weight of 446.46 Da and a net negative charge at neutral pH.

The peptide belongs to the Khavinson family of short peptide bioregulators — molecules hypothesised to interact with chromatin and DNA to modulate gene expression in a tissue‑specific manner. It was developed by Vladimir Khavinson at the Institute of Bioregulation and Gerontology in St. Petersburg, Russia, as a tissue‑specific bioregulator targeting bronchial epithelium.

Mechanism of Action: Epigenetic Modulation and DNA Stabilisation

The biological activity of Bronchogen (20mg) research peptide is hypothesised to involve several intersecting mechanisms:

DNA Stabilisation and Thermostability

Bronchogen has been shown to serve as a DNA‑stabilising agent. A study published in the Bulletin of Experimental Biology and Medicine (2011) demonstrated that Bronchogen increased the melting temperature of DNA from calf thymus and mouse liver by 3.1°C in a narrow range of molar ratios (0.01‑0.055). This increase in melting temperature indicates enhanced DNA thermostability, reflecting how much heat is needed to denature the double‑stranded DNA into single strands. The binding occurs with both strands of DNA (mainly with nitrogen bases) and is considered strong and occasional.

Chromatin Interaction and Epigenetic Regulation

Bronchogen interacts with core histones — H1, H2B, H3, and H4 — at N‑terminal peptide binding motifs. This suggests that Bronchogen might modify chromatin structure, potentially affecting gene accessibility and transcription by controlling how tightly or loosely DNA is wound around these histones. Additionally, Bronchogen binds preferentially with deoxyribooligonucleotides containing CTG sequences, which are targets for cytosine DNA methylation in eukaryotes. This indicates a potential role for Bronchogen in epigenetic regulation — modifying gene expression without altering the DNA sequence — particularly in genes relevant to bronchial epithelial cell function.

Gene Expression Modulation in Bronchial Epithelial Cells

Research has demonstrated that Bronchogen regulates the synthesis of several proteins in cultures of human bronchoepithelial cells, including Ki67, Mcl‑1, p53, CD79, and NOS‑3. It activates the expression of genes coding for bronchoepithelial cell differentiation factors, including Nkx2.1, SCGB1A1, SCGB3A2, FoxA1, and FoxA2, as well as MUC4, MUC5AC, and SftpA. Bronchogen has also been shown to regulate the expression of the cell proliferation marker gene MKI67 (Ki67), various anti‑apoptotic genes such as MCL1, and the tumour suppressor gene TP53 (p53).

Tissue‑Specific Differentiation Stimulation

Bronchogen tissue‑specifically stimulates the expression of differentiation factors in bronchial epithelial cells. The peptide activates the expression of genes coding for bronchoepithelial cell differentiation factors and markers of functional activity, potentially preventing the development of pulmonary pathology.

Key Research Applications for Bronchogen (20mg)

Bronchogen (20mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Respiratory Epithelium & Pulmonary Research

As a peptide that tissue‑specifically stimulates bronchial epithelial cells, Bronchogen is a valuable tool for studying respiratory biology. Research applications include:

• Bronchial Epithelial Cell Cultures: Investigating how Bronchogen influences gene expression, differentiation, and function in bronchial epithelial cells.

• Chronic Obstructive Pulmonary Disease (COPD) Models: In a rat COPD model created by 60‑day intermittent exposure to nitrogen dioxide, Bronchogen decreased neutrophilic inflammation, normalised cellular composition and pro‑inflammatory cytokines, and restored the structure and functional activity of the bronchial epithelium.

• Lung Function Models: Exploring the peptide’s potential to support lung function and epithelial integrity.

• Acute Bacterial Lung Inflammation, Fibrosis, and Toxic Lung Damage: The tetrapeptide AEDL was effective on models of acute bacterial lung inflammation, fibrosis, and toxic lung damage, demonstrating protective effects on bronchial epithelium.

2. Epigenetic & Chromatin Research

As a peptide that interacts with DNA and histones, Bronchogen is a valuable tool for studying epigenetic regulation. Research applications include:

• DNA‑Protein Interactions: Investigating how short peptides bind to DNA and modulate its stability and structure.

• Chromatin Remodelling: Studying how Bronchogen influences chromatin structure and gene accessibility.

• Epigenetic Modulation: Exploring the role of Bronchogen in regulating gene expression through DNA methylation and histone modification.

• Gene Expression Profiling: Investigating how Bronchogen modulates the expression of genes involved in bronchial epithelial cell function, inflammation, and cell proliferation.

3. Inflammation & Anti‑Fibrotic Research

Bronchogen has demonstrated anti‑inflammatory and anti‑fibrotic effects in preclinical models. Research applications include:

• Inflammatory Signalling: Investigating how Bronchogen modulates the expression of inflammatory genes such as CD79 and NOS‑3.

• Pulmonary Fibrosis Models: Studying Bronchogen’s potential to mitigate fibrotic processes in the lungs.

• Cytokine Modulation: Exploring how Bronchogen decreases the activity of neutrophilic inflammation and normalises the profile of pro‑inflammatory cytokines.

4. Cell Proliferation & Differentiation Research

Bronchogen’s effects on genes associated with the cell cycle and apoptosis make it a valuable tool for studying cellular differentiation and proliferation. Research applications include:

• Cell Cycle Regulation: Investigating how Bronchogen modulates the expression of Ki67 (a proliferation marker) and p53 (a tumour suppressor).

• Apoptosis Studies: Exploring the role of Bronchogen in regulating anti‑apoptotic genes such as MCL1.

• Differentiation Factors: Studying how Bronchogen stimulates the expression of differentiation factors in bronchial epithelial cells.

5. Geroprotection & Aging Research

Bronchogen has demonstrated geroprotective effects in preclinical research. Research applications include:

• Age‑Related Respiratory Decline: Investigating the peptide’s potential to preserve lung function with aging.

• Cellular Senescence: Studying how Bronchogen influences age‑related changes in bronchial epithelial cells.

• Tissue Homeostasis: Exploring the role of Bronchogen in maintaining normal structural and functional homeostasis of cell populations.

Chemical & Physical Specifications for Bronchogen (20mg)

Accurate characterisation of Bronchogen (20mg) research peptide is essential for reproducible experimental design.

Note: The 20 mg mass refers to the weight of the peptide in its lyophilised form. Bronchogen is typically supplied as an acetate salt, which enhances aqueous solubility. The peptide is for research use only (RUO). Not for human consumption. Bronchogen is not FDA‑approved for therapeutic use.

Reconstitution & Handling Protocol for Bronchogen (20mg)

Proper reconstitution of Bronchogen (20mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Bronchogen vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Bronchogen vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 20 mg per 2 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Bronchogen into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for Bronchogen (20mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for Bronchogen (20mg)

Why Choose Chinese Grey Peptides for Bronchogen (20mg)?

As an authentic licensed peptide supplier based in the USA, we supply Bronchogen (20mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO). Bronchogen is not FDA‑approved for therapeutic use.

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Bronchogen (20mg) Research Peptide

What is Bronchogen (20mg) research peptide?
Bronchogen (AEDL, T‑33) is a synthetic tetrapeptide (Ala‑Glu‑Asp‑Leu) belonging to the Khavinson family of peptide bioregulators. It is used in research to study respiratory biology, epigenetic regulation, and tissue‑specific gene expression in bronchial epithelial cells.

What is the sequence and molecular weight of Bronchogen?
The sequence is Ala‑Glu‑Asp‑Leu (AEDL). Its molecular formula is C₁₈H₃₀N₄O₉, and its molecular weight is approximately 446.45 g/mol.

What are the primary research applications for Bronchogen?
Bronchogen is used in respiratory epithelium and pulmonary research (COPD models, acute bacterial lung inflammation), epigenetic and chromatin research (DNA stabilisation, histone interaction), inflammation and anti‑fibrotic research, cell proliferation and differentiation studies, and geroprotection and aging research.

 What is the mechanism of action of Bronchogen?
Bronchogen is hypothesised to serve as a DNA‑stabilising agent (increasing melting temperature by 3.1°C), interact with histones H1, H2B, H3, and H4, bind preferentially to CTG sequences, and modulate the expression of genes involved in bronchial epithelial cell function, inflammation, and cell proliferation.

What evidence supports Bronchogen’s effects in COPD models?
In a rat COPD model, Bronchogen decreased neutrophilic inflammation, normalised cellular composition and pro‑inflammatory cytokines, restored the structure of the bronchial epithelium, and increased secretory immunoglobulin A and surfactant protein B.

 What purity does your Bronchogen (20mg) meet?
Our Bronchogen is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Bronchogen (20mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

 How should I store reconstituted Bronchogen?
Reconstituted Bronchogen should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

 Is Bronchogen research peptide legal to purchase in the USA?
Yes. Bronchogen is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: Bronchogen is not FDA‑approved for therapeutic use and has not been evaluated by any Western regulatory agency.

Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

1. Shop Page
2. Bulk Orders Page
3. Contact Page
4. Shipping & Return Page
5. Peptide Purity Page

• PubMed: Peptide regulation of gene expression and protein synthesis in bronchial epithelium
• PubMed duplicate abstract page
• FDA Gram Peptides warning letter .
• Google Product Structured Data
• Rank Math WooCommerce Product Schema

Ships to USA, Canada, UK & Europe

Chinese Grey Peptides supports shipping to the USA, Canada, the United Kingdom, France, Germany, Spain, Sweden, Italy, and Europe where permitted.

Shipping support may include:

• Order processing updates
• Packaging support
• Tracking information where available
• Wholesale shipment planning
• Destination-based shipping review
• Customer support during transit

Shipping times may vary by order size, carrier, destination, customs process, product status, and local requirements.

Why Choose Chinese Grey Peptides?

Chinese Grey Peptides focuses on quality, support, and clear communication. We help buyers review product details before placing an order.

Customers choose us for:

• Professional peptide supplier support
• Small and wholesale order options
• Product documentation support
• HPLC testing information where available
• Mass Spectrum documentation where available
• International shipping support
• Responsive communication
• Clear order assistance before payment

We aim to make the buying process transparent, professional, and documentation-focused.

Important Compliance Notice

Bronchogen 20mg may be regulated differently depending on the country, product type, labeling, intended use, buyer type, and destination. Chinese Grey Peptides does not provide medical advice, dosing instructions, injection guidance, respiratory claims, lung-health claims, COPD claims, bronchial-support claims, immune claims, anti-inflammatory claims, recovery claims, disease claims, treatment claims, or personal-use recommendations.

Customers are responsible for understanding and following all local laws, import rules, prescription requirements, compounding rules, labeling rules, and permitted-use requirements before ordering.

From its origin as a tetrapeptide derived from bronchial mucosa to its well‑characterised role in DNA stabilisation, chromatin interaction, and tissue‑specific gene expression, Bronchogen (20mg) research peptide provides a precisely defined and increasingly valuable platform for advanced respiratory and epigenetic research.

Its ability to increase DNA thermostability by 3.1°C, interact with histones H1, H2B, H3, and H4, and modulate the expression of genes involved in bronchial epithelial cell function—supported by peer‑reviewed research published in the Bulletin of Experimental Biology and Medicine and other authoritative journals—makes it a valuable addition to any laboratory investigating respiratory biology, epigenetics, inflammation, or the molecular mechanisms of tissue repair. By choosing high‑purity, lab‑verified Bronchogen (20mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Bronchogen (20mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Bronchogen delivered directly to your facility in the USA, UK, or Europe.Bronchogen (20mg)