Humanin (10mg) Research Peptide: A Mitochondrial‑Derived Peptide for Neuroprotection & Cytoprotection Studies

If your research investigates the molecular mechanisms of neuroprotection, mitochondrial function, or the biology of aging and cellular stress responses, Humanin (10mg) research peptide offers a precisely defined synthetic tool with a growing body of scientific literature. Humanin is a 24‑amino‑acid peptide encoded within the mitochondrial genome — the first member of a novel class of mitochondrial‑derived peptides (MDPs) discovered in over three decades.

First identified in the early 2000s for its ability to protect neurons against Alzheimer’s disease‑related neurotoxicity, Humanin has since been shown to exert cytoprotective, anti‑apoptotic, and anti‑inflammatory effects across a diverse range of disease models, including stroke, cardiovascular disease, neurodegeneration, and cancer. This makes Humanin (10mg) research peptide a valuable tool for laboratories investigating neurodegeneration, mitochondrial dysfunction, metabolic disorders, and the biology of aging.

At Chinese Grey Peptides, we supply high‑purity Humanin (10mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into mitochondrial signalling, neuroprotection, and aging begin with uncompromising quality.

What Is Humanin and How Does It Work?

To fully appreciate the research potential of Humanin (10mg) research peptide, it is essential to understand its unique mitochondrial origin and the mechanisms by which it exerts its pleiotropic effects.

The 24‑Amino‑Acid Mitochondrial Peptide

Humanin is a 24‑amino‑acid peptide encoded within the mitochondrial 16S ribosomal RNA gene (MT‑RNR2). Its sequence is Met‑Ala‑Pro‑Arg‑Gly‑Phe‑Ser‑Cys‑Leu‑Leu‑Leu‑Leu‑Thr‑Ser‑Glu‑Ile‑Asp‑Leu‑Pro‑Val‑Lys‑Arg‑Arg‑Ala (MAPRGFSCLLLLTSEIDLPVKRRA). The peptide has a molecular formula of C₁₁₉H₂₀₄N₃₄O₃₂S₂, a molecular weight of approximately 2,687.23 g/mol, and a CAS number of 330936‑69‑1. It has a half‑life of approximately 30 minutes in mice.

Humanin is the founding member of a new class of small signalling peptides that originate from the mitochondrial genome — a discovery that challenged the long‑held view that human mitochondrial DNA encodes only 13 essential components of the electron transport chain. Its expression declines with age, coincident with the increased likelihood for the development of Alzheimer’s disease and other age‑related conditions.

Mechanism of Action: Multi‑Target Cytoprotective Signalling

The biological activity of Humanin (10mg) research peptide is mediated through several intersecting pathways:

Anti‑Apoptotic Activity via Bax Inhibition

Humanin’s primary cytoprotective mechanism involves the inhibition of Bax‑dependent apoptosis. It binds to Bax and prevents its translocation from the cytosol to the mitochondria, blocking the conformational change from the inactive to active form. This action suppresses Bax‑dependent apoptosis and has been demonstrated to protect cells from a range of insults, including oxidative stress, serum starvation, and hypoxia.

Anti‑Inflammatory and NF‑κB Modulation

Humanin regulates inflammation by modulating the NF‑κB pathway, leading to reduced expression of pro‑inflammatory cytokines. It possesses anti‑oxidant, anti‑inflammatory, and pro‑apoptotic properties.

Autophagy Induction and Mitochondrial Protection

Humanin is an autophagy inducer that promotes cell survival in the presence of metabolic and oxidative stress. It re‑establishes mitochondrial function and biogenesis. Under stress conditions, Humanin helps maintain mitochondrial function and cell viability, playing a cytoprotective role.

Neuroprotection via Trimeric Receptor Signalling

Humanin protects neurons from the toxic effects of amyloid beta (Aβ) by improving hippocampal acetylcholine levels and attenuating oxidative stress and associated neurotoxicity. It has been shown to activate the ERK1/2, AKT, and STAT3 signalling pathways in the hippocampus. Humanin attenuates Alzheimer‑like cognitive deficits and pathological changes induced by amyloid β‑peptide in rats.

Cytoprotection Across Multiple Cell Types

Beyond neurons, Humanin protects other cells from amyloid β‑induced toxicities and other stresses. It protects RPE cells from oxidative stress‑induced cell death, senescence, and mitochondrial dysfunction.

Key Research Applications for Humanin (10mg)

Humanin (10mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Neurodegenerative Disease & Alzheimer’s Research

The most extensively studied application of Humanin lies in its neuroprotective properties. Research has demonstrated:

• Protection Against Aβ Toxicity: Humanin protects neurons from amyloid beta‑induced toxicity and improves cognitive function in animal models of Alzheimer’s disease

• Neurotransmitter Support: Humanin improves hippocampal acetylcholine levels, which are critical for memory and cognitive function

• Synaptic Plasticity: Treatment with Humanin increases the number of dendritic branches and the density of dendritic spines, and upregulates pre‑ and post‑synaptic protein levels

• Biomarker Potential: Humanin is regarded as a biomarker of mitochondrial stress, and its levels change with aging and neurodegeneration

• Neuroprotection Against Multiple Insults: Humanin suppresses neuronal cell death caused by all known familial Alzheimer’s disease (FAD) mutants and Aβ

2. Aging & Senescence Research

Humanin levels decline with age, and its restoration has been shown to counteract age‑related processes. Research has demonstrated:

• Cognitive Aging: Humanin administration improves cognition in aged mice, and a single nucleotide polymorphism (SNP) in the humanin gene is associated with accelerated cognitive aging

• Longevity: A humanin variant (P3S) is associated with longevity in APOE4 carriers and resists APOE4‑induced brain pathology

• Mitochondrial Protection: Humanin preserves mitochondrial function and cell viability under stressful and senescence conditions

• Age‑Related Diseases: Humanin can be exploited in strategies aiming to counteract processes involved in aging, including cardiovascular disease, neurodegeneration, and cancer

3. Mitochondrial Biology & Metabolic Research

As a mitochondrial‑derived peptide, Humanin is a valuable tool for studying mitochondrial function and metabolism:

• Mitochondrial Protection: Humanin preserves mitochondrial functional integrity

• Metabolic Disorders: Humanin is an autophagy inducer that can promote cell survival in the presence of metabolic and oxidative stress

• Cellular Stress Responses: Humanin exerts its effects by inhibiting pro‑apoptotic factors, including BAX and tBID

• Mitochondrial Biogenesis: Humanin re‑establishes mitochondrial function and biogenesis

4. Cardiovascular & Ischaemic Research

Humanin has demonstrated protective effects in cardiovascular disease models:

• Ischaemia/Reperfusion Injury: Humanin protects against ischaemia/reperfusion injury

• Cardioprotection: Humanin improves outcomes in cardiovascular disease models

• Brain Ischaemia: Humanin may be applied for the management of diseases involving poor metabolic activity, such as mitochondria‑related disorders and brain ischaemia

5. Traumatic Brain Injury (TBI) Research

Humanin ameliorates TBI‑related cognitive impairment by attenuating mitochondrial dysfunction and inflammation. It is a mitochondrial‑derived peptide released by astrocytes that prevents synapse loss in hippocampal neurons.

6. Autophagy & Cellular Stress Research

Humanin is an autophagy inducer that can promote cell survival in the presence of metabolic and oxidative stress. Research applications include:

• Autophagy Pathway Regulation: Investigating the role of Humanin in regulating autophagy pathways for therapeutic application in metabolic disorders

• Oxidative Stress: Humanin possesses anti‑oxidant properties

• Cellular Survival: Under stress conditions, MDP helps maintain mitochondrial function and cell viability

Chemical & Physical Specifications for Humanin (10mg)

Accurate characterisation of Humanin (10mg) research peptide is essential for reproducible experimental design.

Note: The 10 mg mass refers to the weight of the peptide in its lyophilised form. Humanin is typically supplied as a TFA or acetate salt, which enhances aqueous solubility. The peptide contains a cysteine residue, so care should be taken to avoid oxidation. The peptide is for research use only (RUO). Not for human consumption. Humanin is not FDA‑approved for therapeutic use.

Reconstitution & Handling Protocol for Humanin (10mg)

Proper reconstitution of Humanin (10mg) research peptide is critical for maintaining its structural integrity and biological activity. The peptide has a half‑life of approximately 30 minutes in mice, so careful handling is essential.

Step 1 – Preparation

• Bring the sealed Humanin vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Humanin vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 10 mg per 1 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Humanin into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table for Humanin (10mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for Humanin (10mg)

Why Choose Chinese Grey Peptides for Humanin (10mg)?

As an authentic licensed peptide supplier based in the USA, we supply Humanin (10mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• USA‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO). This product is intended for scientific research and experimental use in controlled settings. Not for human or veterinary use.

Researchers across the United States—including those at leading institutions—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Humanin (10mg) Research Peptide

 What is Humanin (10mg) research peptide?
Humanin is a 24‑amino‑acid peptide encoded within the mitochondrial genome — the first member of a novel class of mitochondrial‑derived peptides. It exhibits neuroprotective, cytoprotective, anti‑apoptotic, and anti‑inflammatory properties across a diverse range of disease models.

What is the sequence and molecular weight of Humanin?
The sequence is Met‑Ala‑Pro‑Arg‑Gly‑Phe‑Ser‑Cys‑Leu‑Leu‑Leu‑Leu‑Thr‑Ser‑Glu‑Ile‑Asp‑Leu‑Pro‑Val‑Lys‑Arg‑Arg‑Ala (MAPRGFSCLLLLTSEIDLPVKRRA). Its molecular formula is C₁₁₉H₂₀₄N₃₄O₃₂S₂, and its molecular weight is approximately 2,687.23 g/mol.

What are the primary research applications for Humanin?
Humanin is used in neurodegenerative disease and Alzheimer’s research, aging and senescence studies, mitochondrial biology and metabolic research, cardiovascular and ischaemic research, traumatic brain injury research, and autophagy and cellular stress research.

 What is the mechanism of action of Humanin?
Humanin acts through multiple mechanisms: it inhibits Bax‑dependent apoptosis by preventing Bax translocation from the cytosol to mitochondria, regulates inflammation by modulating the NF‑κB pathway, induces autophagy, and activates the ERK1/2, AKT, and STAT3 signalling pathways.

What is the half‑life of Humanin?
The half‑life of Humanin in mice is approximately 30 minutes.

 What purity does your Humanin (10mg) meet?
Our Humanin is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

 How should I reconstitute Humanin (10mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

How should I store reconstituted Humanin?
Reconstituted Humanin should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Humanin research peptide legal to purchase in the USA?
Yes. Humanin is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United States for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: Humanin is not FDA‑approved for therapeutic use.

 Does Chinese Grey Peptides ship internationally?
Yes. We ship across the UK, Europe (France, Germany, Spain, Sweden, Italy), and the USA from our US warehouse. Wholesale pricing is available for bulk orders.

Humanin (10mg)

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Humanin (10mg)

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Important Compliance Notice

Humanin 10mg may be regulated differently depending on the country, product type, labeling, intended use, buyer type, and destination. Chinese Grey Peptides does not provide medical advice, dosing instructions, injection guidance, neuroprotection claims, anti-aging claims, longevity claims, mitochondrial-health claims, disease claims, treatment claims, athletic-performance claims, or personal-use recommendations.

Customers are responsible for understanding and following all local laws, import rules, prescription requirements, compounding rules, sport-governing rules, labeling rules, advertising rules, and permitted-use requirements before ordering.

From its discovery as the first mitochondrial‑encoded signalling peptide to its well‑characterised role in neuroprotection, anti‑apoptosis, and metabolic regulation, Humanin (10mg) research peptide provides a precisely defined and increasingly valuable platform for advanced mitochondrial and biomedical research.

Its ability to inhibit Bax‑dependent apoptosis, regulate inflammation via the NF‑κB pathway, induce autophagy, and activate the ERK1/2, AKT, and STAT3 signalling pathways—supported by a growing body of peer‑reviewed literature published in PubMed, PMC, and other authoritative journals—makes it a valuable addition to any laboratory investigating neurodegeneration, mitochondrial biology, or the molecular mechanisms of aging. By choosing high‑purity, lab‑verified Humanin (10mg) research peptide from Chinese Grey Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Humanin (10mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Humanin delivered directly to your facility in the USA, UK, or Europe.